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microscope:start [2017/11/18 22:09] – mcmastermicroscope:start [2025/08/04 21:23] (current) – external edit 127.0.0.1
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 +See also: http://microwiki.org
 +
 Since microscopes are a critical instrument this section provides information on both microscope techniques and equipment.  There are already many good sites on microscope techniques so the focus will be more on discussing which ones are relevant for ICs and providing external links to provide more information. Since microscopes are a critical instrument this section provides information on both microscope techniques and equipment.  There are already many good sites on microscope techniques so the focus will be more on discussing which ones are relevant for ICs and providing external links to provide more information.
 +
 +[[:motor:stepper]]
  
 ====== General reccomendations (optical) ======  ====== General reccomendations (optical) ====== 
  
-====== Base microscope ======+===== Base microscope =====
  
 TLDR: TLDR:
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   * Olympus BH is a good surplus option if you need basic brightfield and/or darkfield imaging   * Olympus BH is a good surplus option if you need basic brightfield and/or darkfield imaging
   * Olympus BH2 is a good option if you need more advanced features, but will cost a lot more   * Olympus BH2 is a good option if you need more advanced features, but will cost a lot more
 +  * K2-IND is a good confocal option
 +  * Only use long working distance (LWD) objectives if you need them
 +    * They are expensive
 +    * They have lower resolution
 +    * Oil objectives (almost contacting sample) will have the highest NA (say 1.4) => resolution
 +  * Probe station
 +    * B&L industrial objectives is a good low cost option
 +    * Mitutoyo FS50 or FS60 is a good higher end option
 +      * Can add NWR ezlaze for laser probe station
  
 When I first started seriously looking for equipment (Say around 2010) the Olympus BH / BH2 were pretty good options, with a complete BH costing maybe $300 and the BH2 costing maybe $600. I've heard the market has dried up a bit, but they still may be decent options. Note the BH is a 210 mm finite system while the BH2 uses infinity optics. Therefore, you should not mix parts from the two or image quality will suffer. When I first started seriously looking for equipment (Say around 2010) the Olympus BH / BH2 were pretty good options, with a complete BH costing maybe $300 and the BH2 costing maybe $600. I've heard the market has dried up a bit, but they still may be decent options. Note the BH is a 210 mm finite system while the BH2 uses infinity optics. Therefore, you should not mix parts from the two or image quality will suffer.
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-====== Automation (ie CNC) ======+===== Automation (ie CNC) =====
  
 Summary Summary
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 Some non-CNC microscope chassis may be easier to mod for CNC control. For example, the Olympus BHMJ is a boom mount, which can be easy to re-mount with a stage. Some non-CNC microscope chassis may be easier to mod for CNC control. For example, the Olympus BHMJ is a boom mount, which can be easy to re-mount with a stage.
  
 +===== Camera =====
  
-====== McMaster reference system ======+Things to consider: 
 +  * What resolution do you need? Likely your lowest magnitude objective will drive this 
 +  * Consider how camera RGB pattern will effect resolution 
 +    * I use 4x resolution to compensate 
 +  * How many bits do you need? Most cameras are 8 bit, but 12 or 16 bit will provide better dynamic range 
 +  * Do you have low light requirements? Otherwise you probably don't need a cooled camera 
 + 
 +For Linux software, as of Nov 2017, I believe the MU800 is the only camera with V4L support in Linux (also noting there are MU300 and MD1900 drivers not in mainline). AmScope/TopuTek/ScopeTek does provide libusb drivers though, so you could make a custom application. Finally, note uManager supports misc microscope stages out of box. 
 + 
 +Some people elect to use DSLRs. You can control these using gPhoto2 or similar solutions. 
 + 
 + 
 +===== McMaster reference system =====
  
 My standard system is something like: My standard system is something like:
   * Olympus BH2 core w/ BH2-UMA   * Olympus BH2 core w/ BH2-UMA
 +  * Chassis
 +    * Don't have a consistent option here
 +    * Post mounted (ex: BH2 BHMJ) is easy to use
 +    * The large frame used on prawnscope is relatively popular, but I don't know model number
   * Surplus linear stages   * Surplus linear stages
     * Retrofitted with MDrive17 integrated driver stepper motors     * Retrofitted with MDrive17 integrated driver stepper motors
 +    * Machined mounts
 +  * Mirror mount for leveling
 +    * Full size: large mount (ex: Newport NRC 39) rightside up
 +    * Small / low cost: small mount (ex: Newport MM2) upside down
   * Machinekit on beagle bone black (BBB) as CNC controller   * Machinekit on beagle bone black (BBB) as CNC controller
 +    * Usually with an Adafruit 572 (Proto Cape Kit for BeagleBone & Beagle Bone Black) for connectors and/or level translation
   * Thinkpad T61p controlling the microscope   * Thinkpad T61p controlling the microscope
   * MU800 microscope camera   * MU800 microscope camera
   * Modified v4l Linux driver for the MU800   * Modified v4l Linux driver for the MU800
     * For direct RGB control     * For direct RGB control
-  * pr0ntools (libpano core) for image stitching+  * prawntools (libpano core) for image stitching 
 + 
 +[[https://siliconprawn.org/wiki/doku.php?id=mcmaster|Specific system information here]]
  
  
 ====== SEM ====== ====== SEM ======
 +
 +TLDR:
 +  * Good entry level option (~30 nm): RJ Lee PSEM
 +  * Good entry level option (~7 - 30 nm): a recently maintained ISI SEM
 +  * Modern higher resolution (< 10 nm): expect to pay at least 7k (as of 2017)
  
 A good entry level SEM is the RJ Lee PSEM. They are relatively compact and list for about $2-3k on eBay. For example, my PSEM cost about $2000, including shipping, to get operational. However, the instrument is relatively limited, providing an estimated resolution of only about 30 nm. The Super IIIA, for comparison, has a resolution of about 7 nm. This means a hit of about 18x as much information with a 2D image. A good entry level SEM is the RJ Lee PSEM. They are relatively compact and list for about $2-3k on eBay. For example, my PSEM cost about $2000, including shipping, to get operational. However, the instrument is relatively limited, providing an estimated resolution of only about 30 nm. The Super IIIA, for comparison, has a resolution of about 7 nm. This means a hit of about 18x as much information with a 2D image.
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 Follow the [[http://hugin.sourceforge.net/tutorials/scans/en.shtml|Hugin tutorial for stitching scanner images]]. If you have an XY stage, you can take out the rotation parameter since it should be negligible. Follow the [[http://hugin.sourceforge.net/tutorials/scans/en.shtml|Hugin tutorial for stitching scanner images]]. If you have an XY stage, you can take out the rotation parameter since it should be negligible.
  
-====== Field Stop (FS) and Aperature Stop (FS) ======  
- 
-Properly adjusted can give a real improvement in image quality.  Unadjusted (Mit20x objejective): 
- 
-{{:microscope:as_fs_unadj_mit20x.jpg?300|}} 
- 
-Adjusted (Mit20x objejective): 
- 
-{{:microscope:as_fs_adj_mit20x.jpg?300|}} 
- 
- 
-====== DIY non-optical ======  
- 
-  * [[http://sxm4.uni-muenster.de/stm-en/|STM Construction Kit]] 
-  * [[http://www.e-basteln.de/|STM: A project by Jurgen Muller]] 
-  * http://dberard.com/home-built-stm/ 
  
microscope/start.1511042983.txt.gz · Last modified: 2017/11/18 22:09 by mcmaster