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| microscope:tech_inst:k2_ind [2014/09/09 05:08] – mcmaster | microscope:tech_inst:k2_ind [2018/10/12 04:59] – mcmaster | ||
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| Setup overview: | Setup overview: | ||
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| + | Description of the setup: | ||
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| + | The K2 IND 210mm (Nikon) confocal attachment is mounted on a custom built adjustable microscope stand which includes an ASI XY motorized stage and a linear Z stage.\\ | ||
| + | On the left you can see the stage controller as well as a custom built joystick controller. With the joystick controller, the microscope stages can be\\ | ||
| + | conveniently moved through a regular PC joystick or gamepad (in this case an Xbox gamepad). Besides, the joystick controller passes through stage commands from the PC to the stage.\\ | ||
| + | Below the lamp power supply on the right there is a frequency generator that is connected to the sync input of the K2 IND. Without the frequency generator the spinning disc turned too fast\\ | ||
| + | and caused vibrations that made the acquired image blurry. With the frequency generator, the speed of the spinning disc can be controlled and the vibrations are gone.\\ | ||
| + | On the camera port of the trinocular head we use a 3D printed camera adapter to control the focal length of the camera. On the left of the K2 IND there is an adjustment screw that lets you\\ | ||
| + | set up the internal focus (i.e. you should see the holes of the disc as well as the sample under the microscope clear at the same time). Without the custom 3D printed camera adapter, either\\ | ||
| + | the camera picture or the picture through the binoculars was sharp, but not both at the same time. The camera adapter solves this issue.\\ | ||
| + | For image acquisition an industrial grade DCAM/IIDC FireWire camera is used. On the PC side, the open-source micro-manager software is utilized with a custom written micro-manager camera DeviceAdapter allowing automated stage control, image acquisition, | ||
| Internals: | Internals: | ||
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| ===== Disposition services (Nikon) ===== | ===== Disposition services (Nikon) ===== | ||
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| ====== McMaster systems ====== | ====== McMaster systems ====== | ||
| - | Trying to restore a pair of heavy duty systems pieced together from industrial auctions | + | [[mcmaster: |
| - | [[mcmaster: | + | [[mcmaster: |
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| + | Trying to restore a pair of heavy duty systems pieced together from industrial auctions | ||
| - | [[mcmaster: | ||
| ====== References ====== | ====== References ====== | ||