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microscope:tech_inst:k2_ind [2014/06/22 01:40] – mcmaster | microscope:tech_inst:k2_ind [2018/10/12 04:59] – mcmaster | ||
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- | This is a very popular confocal module | + | The K2 industrial (" |
* 012-00010-AA: | * 012-00010-AA: | ||
* 012-00010-AB: | * 012-00010-AB: | ||
- | Because it is spinning disc type, you do not need any special electronics and can view the image directly in the eyepiece. | + | Because it is spinning disc type, you do not need any special electronics and can view the image directly in the eyepiece. |
Optional "focus finder" | Optional "focus finder" | ||
- | ====== Nikon ====== | + | Specifications: |
+ | |||
+ | * Power: 12 VAC @ 400 mA | ||
+ | * 20 lbs | ||
+ | * 6" wide, 4" tall, 16" long | ||
====== Objectives ====== | ====== Objectives ====== | ||
Line 16: | Line 20: | ||
According to [[http:// | According to [[http:// | ||
- | ^ Vendor ^ Series ^ Type ^ Target microscope ^ Threading ^ Notes ^ | + | ^Vendor ^Series ^Type ^Target microscope ^Threading ^Notes |
- | | Olympus | NeoPlan | | BH2 | M25 | | | + | |Olympus |NeoPlan | |BH2 |M25 | | |
- | | Olympus | LWDMPlan | | | RMS | | | + | |Olympus |LWDMPlan | | |RMS | | |
- | | Olympus | M | | BH | RMS | | | + | |Olympus |M | |BH |RMS | | |
- | | Olympus | MNH | | | RMS | | | + | |Olympus |MNH | | |RMS | | |
- | | Olympus | MNM | | | RMS | | | + | |Olympus |MNM | | |RMS | | |
- | | Olympus | MPL | | | RMS | | | + | |Olympus |MPL | | |RMS | | |
- | | Olympus | MPlan | | | RMS | | | + | |Olympus |MPlan | | |RMS | | |
- | | Olympus | MPLL | | | RMS | | | + | |Olympus |MPLL | | |RMS | | |
- | | Olympus | Neo | | BH | M25 | | | + | |Olympus |Neo | |BH |M25 | | |
- | | Olympus | NeoPlan | | BH? | M25 | | | + | |Olympus |NeoPlan | |BH? |M25 | | |
+ | ====== Nikon ====== | ||
+ | |||
+ | System components: | ||
+ | |||
+ | ^Image ^Vendor ^Part # ^Type ^Description ^Notes | | ||
+ | |{{: | ||
+ | |{{: | ||
+ | |{{: | ||
+ | | |Ushio Inc. |USH-102DH |Bulb |" | ||
+ | | |Nikon |? |Bulb |Xeon |For LH-M100CB-1 | | ||
+ | |{{: | ||
+ | |{{: | ||
+ | | |Nikon |BD PlanAPo %%*%% |Objective |210 mm, B/D, apochromatic | | | ||
===== eBay unit ===== | ===== eBay unit ===== | ||
Objectives: | Objectives: | ||
- | * 1 BD PlanAPo 100/0.90 Nikon Japan 190093 | ||
- | * 1 BD Plan 60/0.80 210/0 Nikon 322933 | ||
- | * 1 BD Plan 20/0.04 210/0 Nikon 421567 | ||
- | * 1 BD Plan 10/0.25 210/0 Nikon 321658 | ||
- | * 1 BD Plan 5/0.1 210/0 Nikon 431584 | ||
+ | * 1 BD PlanAPo 100/0.90 Nikon Japan 190093 | ||
+ | * 1 BD Plan 60/0.80 210/0 Nikon 322933 | ||
+ | * 1 BD Plan 20/0.04 210/0 Nikon 421567 | ||
+ | * 1 BD Plan 10/0.25 210/0 Nikon 321658 | ||
+ | * 1 BD Plan 5/0.1 210/0 Nikon 431584 | ||
===== Markus Kammerstetter unit teardown (Nikon) ===== | ===== Markus Kammerstetter unit teardown (Nikon) ===== | ||
Images copyright him, released CC-BY | Images copyright him, released CC-BY | ||
+ | |||
+ | "The lamphouse is of type " | ||
Setup overview: | Setup overview: | ||
- | {{: | + | \\ |
+ | {{: | ||
- | Internals: | + | Description of the setup: |
- | {{:microscope: | + | The K2 IND 210mm (Nikon) confocal attachment is mounted on a custom built adjustable |
+ | On the left you can see the stage controller as well as a custom built joystick controller. With the joystick controller, the microscope | ||
+ | conveniently moved through a regular PC joystick or gamepad (in this case an Xbox gamepad). Besides, the joystick controller passes through stage commands from the PC to the stage.\\ | ||
+ | Below the lamp power supply on the right there is a frequency generator that is connected to the sync input of the K2 IND. Without the frequency generator the spinning disc turned too fast\\ | ||
+ | and caused vibrations that made the acquired image blurry. With the frequency generator, the speed of the spinning disc can be controlled and the vibrations are gone.\\ | ||
+ | On the camera port of the trinocular head we use a 3D printed camera adapter to control the focal length of the camera. On the left of the K2 IND there is an adjustment screw that lets you\\ | ||
+ | set up the internal focus (i.e. you should see the holes of the disc as well as the sample under the microscope | ||
+ | the camera picture or the picture through the binoculars was sharp, but not both at the same time. The camera adapter solves this issue.\\ | ||
+ | For image acquisition an industrial grade DCAM/IIDC FireWire camera is used. On the PC side, the open-source micro-manager software is utilized with a custom written micro-manager camera DeviceAdapter allowing automated stage control, image acquisition, | ||
+ | |||
+ | Internals: | ||
+ | {{: | ||
===== Disposition services (Nikon) ===== | ===== Disposition services (Nikon) ===== | ||
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Lamp PS: | Lamp PS: | ||
- | {{: | + | {{: |
+ | Labels: | ||
+ | |||
+ | < | ||
+ | MODEL HB-10103AF | ||
+ | Nikon corp. | ||
+ | MADE IN JAPAN | ||
+ | |||
+ | FOR 100W | ||
+ | SUPER HIGH | ||
+ | PRESSURE | ||
+ | MERCURY LAMP | ||
+ | |||
+ | CASE | ||
+ | AC IN | ||
+ | 100-120V~ | ||
+ | 50/60Hz 3.5A | ||
+ | </ | ||
====== Leitz ====== | ====== Leitz ====== | ||
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Overview: | Overview: | ||
- | {{: | + | {{: |
- | {{: | + | |
- | K2 ind confocal module: | + | K2 ind confocal module: |
{{: | {{: | ||
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{{: | {{: | ||
+ | |||
+ | ====== McMaster systems ====== | ||
+ | |||
+ | [[mcmaster: | ||
+ | |||
+ | [[mcmaster: | ||
+ | |||
+ | Trying to restore a pair of heavy duty systems pieced together from industrial auctions | ||
====== References ====== | ====== References ====== | ||
- | * User manual: [[http:// | + | * User manual: [[http:// |
+ | * Nikon AZ-FL Epi-Fluoresence Attachment Instructions [AZ-FL]: [[http:// | ||
+ | * C-SHG1 manual: [[http:// | ||
+ | |||
+ | \\ | ||